PCR Based RFLP Genotyping of Bovine Lymphocyte Antigen DRB3.2 in Iranian Holstein Population

Polymorphism of Bovine Lymphocyte Antigen DRB3.2 in Holstein Bulls of Iran Using PCR-RFLP

The Holstein bulls (n=50) were genotyped for bovine lymphocyte antigen (BoLA-DRB3.2) alleles by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Genomic DNA was extracted from bull semen using phenol-chloroform method. A two-step PCR was conducted in order to amplify a 284 base-pair fragment of the target gene. Amplicons were digested by RsaI, HaeIII and BstyI ...

polymorphism of bovine lymphocyte antigen drb3.2 in holstein bulls of iran using pcr-rflp

the holstein bulls (n=50) were genotyped for bovine lymphocyte antigen (bola-drb3.2) alleles by polymerase chain reaction and restriction fragment length polymorphism (pcr-rflp). genomic dna was extracted from bull semen using phenol-chloroform method. a two-step pcr was conducted in order to amplify a 284 base-pair fragment of the target gene. amplicons were digested by rsai, haeiii and bstyi ...

Analysis of relationship between bovine lymphocyte antigen DRB3.2 alleles, somatic cell count and milk traits in Iranian Holstein population.

The major histocompatibility complex (MHC) is a gene complex closely linked to the vertebrate immune system due to its importance in antigen recognition and immune response to pathogens. To improve our understanding of the MHC and disease resistance in dairy cattle, we gathered 5119 test day records of somatic cell count (SCC) and performance traits of 262 Holstein dairy cows to determine wheth...

Frequency of Bovine Lymphocyte Antigen DRB3.2 Alleles in Sarabi Cows

Genotyping of Pasteurella multocida ovine and bovine isolates from Iran based on PCR-RFLP of ompH gene

Pasteurella multocida (P. multocida), A Gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. The outer membrane of Gram-negative bacteria contains of many different protein in very high copy numbers. One of the major outer membrane, the H proteins have functional as high immunogenicity and antigenicity. In this study ...